P-90: Effect of Phosphodiesterase Type3 Inhibitor, Cilostamide, on The Developmental Competence of Ovine OocytesIsolated by Glucose 6-Phosphate Dehydrogenase Activity

Authors

  • Hajian M
  • Hosseini SM
  • Ostadhosseini S
  • Rahmani HR
Abstract:

Background: The developmental competence of oocytes matured in vitro (IVM) is yet far below than in vivo counterparts. Recent studies suggest that the asynchrony between nuclear/cytoplasmic maturation and the initial low/heterogeneous quality of oocytes are the most important factors affecting IVM success. We investigated whether selection of growing oocytes (based on their glucose 6- phosphate dehydrogenase (G6PDH) activity) and chemical induction of nuclear/cytoplasmic synchrony (through transient inhibition of meiosis resumption with cilostamide) can improve developmental competence of sheep oocytes Materials and Methods: Abattoir-derived oocytes were stained with 26μM BCB for 45 minutes to isolate growing (high G6PDH activity) and fully grown (low G6PDH) oocytes according to their differential capacities in breaking BCB and retaining colorless (BCB-) or blue (BCB+) ooplasm, respectively. Then, BCB- and BCB+ oocytes were incubated with 1 μM cilostamide for 6h before culture in normal IVM medium. Then, matured oocytes were used for embryo development assessment using parthenogenetic activation Results: Cilostamide delayed meiotic progression in BCB- ovine oocytes. The cleavage,blastocyst and hatching rates in BCB- oocytes that treated by cilostamide were higher than that of control group, although these increases were not statistically significant Conclusion: We concluded that increase of ovine oocyte cAMP concentration during two-step culture partially improves yield and quality of in vitro embryo. This also may suggest that phosphodiesterase type 3-mediated inhibition of cAMP activity is not the only mechanism that controls the process of nuclear maturation in ovine oocyte.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

the effect of e-64 on the developmental competence of sheep cocs during in vitro maturation

in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.

P-144: Glucose-6-Phosphate Dehydrogenase Activity in Ovine Oocytes in Association with Developmental Characteristics and Expression of Bax Gene

Background: Recent studies have revealed that oocyte competence can be assessed through the presence of the glucose-6-phosphate dehydrogenase (G6PDH) enzyme, as indicated by brilliant cresyl blue (BCB), a dye that can be degraded by G6PDH. In the present study, we aimed to examine the validity of BCB test to select developmentally competent oocyte in ovine and its association with stage-specifi...

full text

INHIBITION OF HUMAN ERYTHROCYTE GLUCOSE 6-PHOSPHATE DEHYDROGENASE ACTIVITY BY DEHYDROEPIANDROSTERONE AND RELATED STEROIDS.

The inhibitory effects of several steroids on G6PD activity using intact erythrocytes are reported. Incubation of whole blood with dehydroepiandrosterone (DHEA) resulted in 42% and 12% inhibition in the enzyme activity in the presence and absence of oxygen, respectively. Addition of epinephrine and/or aminophylline into the incubation medium caused further enzyme inhibition suggesting a po...

full text

P-106: Effect of Cilostamide on Meiotic Arrestof Ovine Oocytes

Background: in vitro maturation (IVM) of oocytes has increasing potential applications in assisted reproductive technology (ART), during which germinal vesicle (GV) oocytes cultured in vitro to produce mature (MII arrested) ones. Although functional, IVM oocytes have low developmental competence compared to in vivo matured oocytes; possibly because IVM comprises asynchrony between nuclear and c...

full text

the effect of lead on erythrocyte glucose-6-phosphate dehydrogenase activity in rats

conclusions the results of this study showed that exposure to lead increased the activity of g6pd in the rat erythrocytes, perhaps resulting in an up regulation of the enzyme to detoxify lead. materials and methods in this study 14 albino rats were divided into two groups of seven animals. the treated group was exposed to 2% lead acetate in the drinking water during eight weeks. the control gro...

full text

Protective Effect of Quercetin on Oxidative Stress in Glucose-6-Phosphate Dehydrogenase-Deficient Erythrocytes in Vitro

Glucose-6-phosphate dehydrogenase (G6PD) deficient subjects are vulnerable to oxidative stress. Quercetin, a flavonoids, has been employed as a potent oxygen-free radical scavenger in order to assess the protective effects of quercetin against H2O2-induced oxidative damage in G6PD-deficient and normal human erythrocytes. Erythrocytes of G6PD-deficient (n = 10) and normal (n = 10) subjects were ...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 8  issue 2.5

pages  104- 104

publication date 2014-07-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023